IDA NUPharose HP

Product Name:	IDA NUPharose HP
Alternative Name:	IDA NUPharose HP
Catalog Number:	NRPB109

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Description

Matrix ：Highly cross-linked agarose, 6%

Ligand ：Iminodiacetic acid, ~18 μmol Ni2+/mL (capacity)

Particle size range ^a： 20~50 μm

Particle size, d50 ：~35 μm

Dynamic binding capacity, DBC_10% ^b ：≥50 mg/mL his-tagged protein/mL

Recommended operating flow velocity^c ：60~150 cm/h

Maximum flow velocity^d：300 cm/h，0.5 MPa

pH stability ：4~8.5 (recommended operating, pH), 3~12 (long-term), 2~14 (short-term)

Chemical stability ：Stable to common aqueous buffers: 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol.

Storage & Shipping ：20% ethanol, 2~30 ℃

Shelf life ：5years

Note: ^aMore than 90% of the microspheres by volume are in this size range; ^bDynamic binding capacity at 10% breakthrough for E. coli expressed recombinant Staphylococcus aureus protein A with His-tag with a residence time of 6 mins; ^cThe flow rate can meet the use conditions of 2-6 mins residence time for various types of columns; ^d1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C.